Introduction:

HEPES

Chemical Name：HEPES

CAS #：7365-45-9

Appearance: White crystal powder

Molecular Formula：C8H18N2O4S

Molecular Weight：238.31

Packing: 25kg plastic drum

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES(7365-45-9)) is a zwitterionic organic buffer used in cellular and molecular biology applications.

HEPES(7365-45-9) is frequently employed in isoelectric focusing and cell culture applications. This buffer has a useful pH range of 6.8 to 8.2.

Product Name: HEPES

Product Grade: Molecular Biology Grade

Alternative Names: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid; 4-(2-Hydroxyethyl)piperazine-1-ethane-sulfonic acid; HEPES Free Acid; HEPES Buffer; C8H18N2O4S

Storage: Room Temperature, Protected From Moisture

HEPES(7365-45-9) (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers. HEPES(7365-45-9) is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES(7365-45-9) is like water in that its dissociation decreases as the temperature decreases. This makes HEPES(7365-45-9) a more effective buffering agent for maintaining enzyme structure and function at low temperatures. Lepe-Zuniga et al. reported a phototoxicity of HEPES(7365-45-9) when exposed to ambient light by the production of hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. It is therefore strongly advised to keep HEPES(7365-45-9)-containing solutions in darkness as much as possible.

HEPES(7365-45-9) has the following characteristics:

pKa1 (25 °C) = 3

pKa2 (25 °C) = 7.5

Useful pH range = 2.5 to 3.5 or 6.8 to 8.2

Certificate of Analysis: